Study snapshot

Temperature stress produced the main package-function signal.

Abstract

This case study describes how ARE Labs tested an anonymized cosmetic serum wand package under controlled temperature storage and simulated altitude exposure. The study separated temperature-related package behavior from risks associated with pressure change.

Purpose

The study helped the manufacturer determine whether storage temperature or transport-related pressure change was more likely to affect package integrity, product presentation, and applicator usability.

Climate set210 units

Three anonymized variants were stored across five temperature conditions for 226 hours.

Altitude set54 units

The same package format was exposed to three simulated altitude conditions in a vacuum chamber.

Main signal60 deg C

The highest temperature condition produced bent wands and wiper displacement.

Findings

Climate storage showed no measurable weight loss at -30 deg C or 5 deg C, but weight loss appeared at 23 deg C and increased at elevated temperatures. The 60 deg C condition produced the clearest package-function concern: bent wands and displaced wipers during opening. Simulated altitude exposure at 18,000 ft, 26,000 ft, and 40,000 ft did not produce leakage in the tested units.

Study question

A cosmetic serum package may appear stable on the bench but behave differently after storage, shipping, or consumer travel. For a wand-and-wiper applicator, package integrity involves more than leakage. The cap, tube, wand, brush, and wiper must continue working together after environmental exposure so the package opens cleanly and dispenses a controlled amount of serum.1,2

The manufacturer needed data showing how three anonymized variants would perform under conditions related to storage, shipping, and use. A simple pass/fail leak check could not answer the full product-development question. Even without obvious external leakage, a displaced wiper, bent wand, or product buildup on the applicator could affect usability.1,2

Why ARE Labs used two environmental studies

ARE Labs designed two complementary studies to separate temperature effects from pressure-change effects. The climate storage study examined the formulation-package system under cold, room-temperature, warm, and high-temperature conditions. The altitude simulation study examined how the package responded to pressure differentials associated with transport or use at elevation.2,3,4

Separating these effects was important because cosmetic package evaluations often emphasize temperature. Heat and cold can affect viscosity, evaporation, plastic components, seals, and usability. Altitude may receive less attention, but pressure changes can matter for packages containing liquid, headspace, closures, applicators, or other internal components. ASTM D6653/D6653M provides public standards context for evaluating high-altitude package effects by vacuum method. FDA cosmetic materials provide context for the product category but do not imply FDA approval.2,3,4

Climate storage simulation

The climate study included 210 total samples across three anonymized product variants. Each variant contributed 70 samples, with 14 samples assigned to each of five temperature conditions: -30 deg C, 5 deg C, 23 deg C, 40 deg C, and 60 deg C. Samples remained in storage for 226 hours, and observations were recorded every 24 hours.1

At each observation interval, ARE Labs evaluated one sample immediately after removal from storage and another after 4 hours at room temperature. Samples were weighed before and after exposure. The inspection covered the package exterior, neck and thread area, internal visual indicators, wand condition, brush appearance, crystallization, product accumulation on the wand, and wiper seating.1

This approach captured both immediate effects of environmental stress and behavior after a short recovery period. The distinction was most important at low temperature, where the serum was temporarily frozen immediately after removal but thawed during the room-temperature hold. Evaluating the full applicator system also prevented the package from being treated as a container alone.1

Table 1Study design summary for the anonymized package-integrity evaluation.1,2
StudyProduct categoryStudy sizeConditions testedObservation planPrimary purpose
Climate storage simulationCosmetic eyelash serum in wand-and-wiper package210 total units-30 deg C, 5 deg C, 23 deg C, 40 deg C, 60 deg CObservations every 24 hours over 226 hours; immediate and 4-hour room-temperature observationsEvaluate temperature effects on formulation-package behavior, mass change, and usability
Altitude leak-resistance simulationCosmetic eyelash serum in wand-and-wiper package54 total unitsSimulated 18,000 ft, 26,000 ft, 40,000 ftImmediate and 4-hour post-exposure inspectionsEvaluate leak resistance and package behavior under simulated pressure-change conditions

Climate storage produced the main signal

The climate study showed a clear temperature-related pattern. Samples tested at -30 deg C and 5 deg C had no measurable weight loss. Weight loss was observed at 23 deg C and above, with a maximum observed loss of 15 mg across the three anonymized variants at 23 deg C. Maximum observed losses increased at 40 deg C and 60 deg C, with the summarized report data showing elevated-temperature values from about 51 mg to 95 mg.1

Figure 1Maximum observed weight loss by storage temperatureGrouped bars compare three anonymized variants from the climate storage report.
Table 2Climate storage maximum observed weight loss.1
Storage temperatureVariant A max lossVariant B max lossVariant C max lossArticle interpretation
-30 deg C0 mg0 mg0 mgNo measurable weight loss; temporary freezing observed immediately after removal
5 deg C0 mg0 mg0 mgNo measurable weight loss
23 deg C15 mg15 mg15 mgWeight loss began at room temperature
40 deg C93 mg52 mg51 mgElevated-temperature weight loss; increased product accumulation; isolated crystallization
60 deg C88 mg95 mg90 mgHighest functional concern; bent wands and wiper displacement

One 40 deg C Variant A value was tied to a single unit; excluding that unit, the Variant A maximum at 40 deg C was 46 mg.

The qualitative observations added important context to the mass measurements. At 5 deg C and 23 deg C, the products generally behaved normally, although product occasionally accumulated on the wand. Wand accumulation became more pronounced at 40 deg C, where isolated crystallization was also observed. The clearest package-function concern occurred at 60 deg C: wands bent, and wipers became displaced from the package body during opening.1

The wiper became the critical component

The wiper was particularly important because it controlled how much serum remained on the wand. When it stayed seated and worked normally, it removed excess product. When the wiper became displaced, the wand retained more serum and appeared visibly overloaded.1

This finding moved the interpretation beyond a narrow leak check and toward package function. Although the tube could appear mostly intact from the outside, the relationship among the internal components had changed. For R&D, quality, and product-management teams, that was the actionable signal: the product-package system needed to be assessed as a working applicator, not simply as a sealed container.1

Table 3Qualitative findings used in article interpretation.1,2
ConditionKey observation usedInterpretation used
-30 deg CSerum froze immediately after removal but thawed after 4 hours at room temperatureTemporary cold-temperature usability issue; not treated as persistent package failure
5 deg CSamples generally behaved normallyLower-temperature storage did not produce the main risk signal
23 deg COccasional product accumulation on wand; weight loss observedEarly temperature-related change
40 deg CMore pronounced wand accumulation; isolated crystallizationElevated-temperature effects became more visible
60 deg CBent wands; wipers detached or became stuck on wandMain package-function concern affecting usability
Simulated altitude exposureNo leakage observed at tested altitudesAltitude was not the primary risk driver for this package in the tested conditions

Altitude exposure did not produce the same pattern

The altitude study included 54 total samples across the same three anonymized product variants. Each variant contributed 18 samples, with six samples exposed at each simulated altitude: 18,000 ft, 26,000 ft, and 40,000 ft. Testing used a sealed vacuum chamber. ARE Labs inspected the samples immediately after exposure and again after 4 hours at room temperature and pressure.2,3

All tested samples passed inspection at both post-exposure time points. No serum leakage was observed, and weight loss remained minimal. This negative finding still helped the manufacturer narrow the risk question. For this package, pressure-change exposure did not reproduce the package-function changes observed during elevated-temperature storage.2,3

Product-development implication

  • Elevated-temperature storage deserved more attention than altitude exposure for this specific product-package configuration.1,2
  • The package needed to be interpreted as a system because the most important finding involved the wand and wiper relationship.1
  • The cold-temperature observation benefited from immediate and 4-hour checks because the serum froze after removal and then thawed at room temperature.1

The documented results do not support broad conclusions about every wand-and-wiper package or cosmetic serum formulation. For this specific anonymized configuration, elevated-temperature storage produced a stronger risk signal than simulated altitude exposure. The cold-temperature result also showed why recovery timing matters: observing a frozen product immediately after exposure has a different product-development meaning than observing that it thawed after 4 hours at room temperature.1,2

For similar programs, inspection criteria should reflect how the product is actually used. If the consumer experience depends on a component remaining seated, an applicator staying straight, or product loading remaining controlled, the protocol should evaluate those behaviors alongside mass change and visible leakage.1,2

Summary

The study found that elevated-temperature storage, particularly at 60 deg C, produced a stronger risk signal for this anonymized package than simulated altitude exposure. This distinction helped the client focus development work on package function, wand-and-wiper interaction, and temperature-related product presentation. ARE Labs connected the study design, inspections, data review, and interpretation to the measured evidence.1,2

Why ARE Labs

ARE Labs connects technical topics to practical study design, method selection, controlled aerosol work, and reportable evidence without turning technical pages into sales pages.

Reviewed byJamie Balarashti (25 yrs - cascade & inhalation methods) - Weston Schaper (7 yrs - real-time sizing & nanoparticle work)
QualityDocumented study records
900+Studies Performed
17+Years in operation
300+Clients supported
Sources

References and study evidence

01Anonymized ARE Labs climate storage simulation reportinternal sourceARE LabsinternalNon-publicNon-public study report used for temperature conditions, mass-change data, and package-function observations.
02Anonymized ARE Labs altitude leak-resistance reportinternal sourceARE LabsinternalNon-publicNon-public study report used for simulated altitude conditions and post-exposure observations.
03ASTM D6653/D6653M-13(2021): Standard Test Methods for Determining the Effects of High Altitude on Packaging Systems by Vacuum Methodstore.astm.org ->ASTM InternationalstandardPublicPublic standards context for vacuum-method evaluation of high-altitude effects on packaging systems.04Cosmetic Productsfda.gov ->U.S. Food and Drug AdministrationgovernmentPublicPublic category context for cosmetic products; not used as approval evidence.

Practical questions

Q.How do you test cosmetic package integrity under storage conditions?
A.A storage study exposes units to defined temperature conditions, tracks mass change, and inspects for leakage, crystallization, component movement, applicator performance, and changes in usability.1
Q.Why test cosmetic products at altitude?
A.Altitude simulation helps evaluate whether pressure changes associated with transport or use at elevation may cause leakage, package deformation, or changes in product presentation.2,3
Q.What did this cosmetic package study find?
A.Climate storage produced the main findings. Weight loss began at 23 deg C and increased at elevated temperatures, while exposure at 60 deg C produced bent wands and displaced wipers. Simulated altitude exposure did not cause leakage in the tested units.1,2
Next step

Discuss a related study

Bring the product format, study question, and available evidence to a project scoping conversation.

Request a quote