Testing services

Bioaerosol Testing Services

Independent chamber, in-duct, and field studies that measure how much viable airborne microbial load a device, a room, or a procedure actually removes — or releases.

Testing services

Bioaerosol Testing Services — key facts

Laboratory
ARE Labs (Aerosol Research and Engineering Labs), an independent third-party testing laboratory
What is measured
Viable airborne microorganism reduction, expressed as log reduction against a device-off control
Study formats
Controlled chamber, in-duct single-pass, and field deployment
Standards referenced
ASTM E2720, ASTM E2721, ASTM E3135, ASHRAE 185.1, ASHRAE 185.2, ASHRAE 241, ISO 16000-36, ISO 16000-37, CDC/NIH BMBL
Endpoints
Culture (CFU), qPCR, and ddPCR, selected against the claim being supported

What does Bioaerosol Testing Services cover?

Bioaerosol testing answers one question: when live microorganisms are suspended in air, how many survive the thing being tested? That thing might be an air cleaner, a UV fixture, an in-duct filter bank, a mask, a detector, or a procedure that could release organisms into a room.

What separates a bioaerosol study from ordinary microbiology is that the organism has to stay airborne, viable, and countable long enough to be measured. Generating a stable challenge aerosol at a known concentration, holding temperature and relative humidity steady, sampling without killing what is captured, and running a device-off control in the same chamber on the same day — that apparatus is the work. It is also why bioaerosol results from different laboratories so often disagree.

Which study do you need?

If the situation is…The study isWhat the report contains
A room air cleaner or UV fixture needs a defensible reduction claimBioaerosol Efficacy Testing RoomLog reduction vs. time against a device-off control, per organism
A device sits in a duct or air handler and treats air in a single passInline Bioaerosol ReductionSingle-pass reduction at rated flow, upstream vs. downstream
A detector, sampler, respirator, or barrier must be proven against a known airborne loadDevice Challenge AerosolResponse or penetration across a controlled concentration range
Equipment or a procedure may emit organisms rather than remove themBioaerosol Risk AssessmentRelease pathways, exposure estimates, and mitigation comparison

Chamber vs. in-duct: which claim is being made

The distinction that trips people up most often is chamber versus in-duct. A chamber study supports a claim about a space — how much airborne microbial load drops in a room over time. An in-duct study supports a claim about the equipment — how much load is removed in a single pass at rated airflow.

The two produce different numbers because they measure different things, and a marketing claim that blends them does not survive regulatory scrutiny. Which published standards frame a given study depends on the device and the claim; ARE Labs treats the standards clusters listed on this page as a citation set for protocol design rather than as methods that automatically govern a bioaerosol result.

How ARE Labs runs the study

Stage 01

Challenge design

Organism panel, concentration range, and carrier fluid chosen against the claim being supported and the standard it must satisfy.

Stage 02

Controlled generation

Nebulized or dry-dispersed challenge into a conditioned chamber or duct, with airflow, temperature, and relative humidity logged throughout.

Stage 03

Sampling and endpoints

Viable samplers with culture, qPCR, or ddPCR endpoints, timed to the decay curve rather than to a fixed clock.

Stage 04

Controls and reporting

Device-off natural-decay control in the same chamber and session, then reduction reported against that control, not against zero.

Standards and regulatory context

Common questions

How long does a bioaerosol study take?

A bioaerosol chamber efficacy study is scheduled around the number of organisms and conditions in scope, because each one needs its own session plus its paired device-off control. Protocol development, when the claim is novel, is usually the longer part of the schedule.

Which organisms can a bioaerosol challenge use?

Organism panels are selected against the claim and the applicable biosafety level. When a specific organism is named in the target standard, ARE Labs works to that organism.

What does a client need to send to ARE Labs?

The device as a customer would receive it, its rated operating settings, and the exact claim wording intended for publication. The claim wording matters more than most clients expect, because it determines the study design.

Can a bioaerosol study be run to a specific standard?

Yes. The standard should be named up front, because it changes the chamber configuration, the organism panel, and the sampling schedule. Retrofitting a completed study to a standard afterwards rarely works.

What is the difference between chamber and in-duct bioaerosol testing?

A chamber study measures how much airborne microbial load drops in a room over time and supports a claim about a space. An in-duct study measures how much load is removed in a single pass at rated airflow and supports a claim about the equipment. The two produce different numbers because they measure different things.

Related reading

Not sure which study fits? Send the claim you want to make and ARE Labs will tell you what it takes to support it.

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